|
KMID : 0370219950390060661
|
|
Yakhak Hoeji
1995 Volume.39 No. 6 p.661 ~ p.665
|
|
|
Protein kinase C-mediated Stimulatory Effect of Ginsenoside-Rg1 on the Proliferation of SK-HEP-1
|
|
공희진/Kong HJ
이광열/정은아/이유희/김신일/이승기/Lee KY/Chung EA/Lee YH/Kim SI/Lee SK
|
|
|
Abstract
|
|
|
|
Ginsenoside-Rg1 (G-Rg1) has been shown to stimulate DNA synthetic activity in SK-HEP-1 cells. This study was therefore designed to determine in SK-HEP-1 cells whether the stimulatory effect of G-Rg1 may be mediated by protein kinase C (PKC) which is known to play a key role in the signal transduction pathway leading to the cell proliferation. Using the in situ PKC assay method, the PKC enzyme activity was determined in SK-HEP-1 cell cultures in response to G-Rg1, at 3X10-5M or phorbol 12-myristate 13-acetate (PMA) at 10-6M which increased the enzyme activity by 1.5- and 7-fold, respectively. Furthermore, G-Rg1, was also able to synergistically increase the enzyme activity by 11-fold in the cell cultures in the presence of PMA. These stimulatory effects of G-Rg1, or PMA on the DNA synthetic activity and the PKC activity were abolished by a specific PKC inhibitor, GF109203X, These results suggest that the stimulatory effect of G-Rg1 on the DNA synthetic activity may be partly due to stimulation of PKC-mediated signal transduction pathway leading to the proliferation of SK-HEP-1 cells.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|